@article{rachdi_regulated_2020, title = {Regulated expression and function of the {GABAB} receptor in human pancreatic beta cell line and islets}, volume = {10}, issn = {2045-2322}, url = {https://www.nature.com/articles/s41598-020-69758-6}, doi = {10.1038/s41598-020-69758-6}, abstract = {Abstract G protein-coupled receptors are seven transmembrane signaling molecules that are involved in a wide variety of physiological processes. They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named {ECN}90 expresses both subunits ( {GABBR}1 and {GABBR}2 ) of the metabotropic {GABA} B receptor compared to human islet. In {ECN}90 cells, baclofen, a specific {GABA} B receptor agonist, inhibits {cAMP} signaling causing decreased expression of beta cell-specific genes such as {MAFA} and {PCSK}1, and reduced insulin secretion. We next demonstrated that in primary human islets, {GABBR}2 {mRNA} expression is strongly induced under {cAMP} signaling, while {GABBR}1 {mRNA} is constitutively expressed. We also found that induction and activation of the {GABA} B receptor in human islets modulates insulin secretion.}, pages = {13469}, number = {1}, journaltitle = {Scientific Reports}, shortjournal = {Sci Rep}, author = {Rachdi, Latif and Maugein, Alicia and Pechberty, Severine and Armanet, Mathieu and Hamroune, Juliette and Ravassard, Philippe and Marullo, Stefano and Albagli, Olivier and Scharfmann, Raphael}, urldate = {2021-10-26}, date = {2020-12}, langid = {english}, keywords = {{WP}5}, }